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KMID : 1094720080130020217
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 2 p.217 ~ p.223
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Identification and characterization of a pigment-producing denitrifying bacterium
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Kim Jeong-Bo
Cho Kyoung-Sook
Jeong Soo-Kyoung
Nam Soo-Wan
Jeong Hyun-Do
Kim Joong-Kyun
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Abstract
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Herein, a denitrifying bacterium that produced greenish fluorescent pigment under aerobic conditions was accidentally isolated from municipal sewage sludge. Using 16S-rDNA sequence analysis, we identified the isolate as Pseudomonas aeruginosa R12, with 100% similarity. We achieved the highest pigment production rate (1.36 mg/L/h) in a 1-L bioreactor under aerobic conditions, using the optimal culture parameters determined in this study: 37°C, pH 8.0, 200 rpm, 5 wm aeration, and medium containing succinate and (NH4)2SO4. The pigment was not a secondary metabolite and had no antibacterial activity on its co-isolates. Under anaerobic conditions, the isolate produced mainly N2 and behaved as a strong denitrifier, displaying synergistic denitrification with co-isolated denitrifiers. To our knowledge, herein we have described the first instance in which P. aeruginosa R12 produces a fluorescent pigment under aerobic conditions. This newly-isolated strain therefore shows potential as a commercial resource for natural pigment.
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KEYWORD
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pigment production, denitrifier, characterization, culture condition, Pseudomonas aeruginosa R12
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